Journal: Human reproduction (Oxford, England)

Article Title: miR-503, a microRNA epigenetically repressed in endometriosis, induces apoptosis and cell-cycle arrest and inhibits cell proliferation, angiogenesis, and contractility of human ovarian endometriotic stromal cells.

doi: 10.1093/humrep/dew217

Figure Lengend Snippet: Figure 4 Effects of miR-503 on the relative protein expressions of downstream target molecules in ECSCs. (A) Cyclin D1. (B) Bcl-2. (C) Rho A. (D) ROCK1. (E) ROCK2. (F) VEGF-A. (G) Representative data of the western blot analysis for cyclin D1, Bcl-2, Rho A, ROCK1 and ROCK2. The protein levels of cyclin D1, Bcl-2, Rho A, ROCK1 and ROCK2 were assessed by performing a western blot analysis. GAPDH was used for normaliza- tion of protein expression. The VEGF-A protein levels in the culture media of two-dimensionally cultured ECSCs were determined by an ELISA. Total protein extracted from a conventional two-dimensional culture of ECSCs was used for the detection of cyclin D1 and Bcl-2. Total protein extracted from a three-dimensional collagen gel culture of ECSCs was used for the detection of Rho A, ROCK1 and ROCK2. Data are presented as percentage of control and are mean ± SD, n = 3. *P < 0.05, Student’s t-test. Bcl-2, B-cell lymphoma/leukemia-2; ECSCs, endometriotic cyst stromal cells; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; Rho A, Ras homology A; ROCK, Rho-associated coiled-coil-forming protein kinase; VEGF-A, vascular endothelial growth factor A.

Article Snippet: The concentration of VEGF-A was determined using ELISA kits (Human VEGF Immunoassay, R&D Systems, Minneapolis, MN, USA).

Techniques: Western Blot, Expressing, Cell Culture, Enzyme-linked Immunosorbent Assay, Control

Journal: Regenerative Therapy

Article Title: Cryopreserved cultured epithelial allografts for pediatric deep partial dermal burns: Early wound closure and suppression of scarring

doi: 10.1016/j.reth.2017.03.002

Figure Lengend Snippet: Levels of growth factors, cytokines and MMP in cryopreserved-allo CEG. The mean values for cryopreserved allo-CEG, calculated from three independent experiments. In cryopreserved allo-CEG, the concentration of IL-1α was ten times the concentrations of other components ( P > 0.01). Although IL-1α is followed by VEGF > TGF-β > TGF-α, there were higher levels in cryopreserved-CEG. KGF, which is not produced by keratinocytes, was not detected. Concentration of MMP-1 and HGF in cryopreserved allo-CEG: Although MMP-1, which suppresses scarring, was contained in cryopreserved allo-CEG. HGF, which is not produced by keratinocytes, was not detected.

Article Snippet: [ELISA Kits Used]: Human IL-1α: Life Technologies (IL-1α ELISA Kit), human IL-1β: Life Technologies (Human IL-1β ELISA Kit), human/mouse PDGF-AA: R&D Systems (Quantikine ® Human/Mouse PDGF-AA Immunoassay), human TGF-α: R&D Systems (Quantikine ® Human TGF-α Immunoassay), human TGF-β1: R&D Systems (Quantikine ® Human TGF-β1 Immunoassay), human VEGF: Life Technologies (Human VEGF ELISA Kit), human KGF: R&D Systems (Quantikine ® Human KGF Immunoassay), Human b-FGF: Life Technologies (Human FGF Basic ELISA Kit): R&D Systems ® (Quantikine ® Human FGF basic Immunoassay), human IL-6: R&D Systems (Quantikine ® Human IL-6 Immunoassay), human Pro-MMP-1: R&D Systems (Quantikine ® ELISA Human Pro-MMP-1), human HGF: Life Technologies (Human HGF ELISA Kit).

Techniques: Concentration Assay, Produced